Separation of Poliovirus Specific Rna Structures

increased significantly when transducing mixtures containing the wild type phage are plated with anti­ phage serum. The second is that transduction by ts mutants at the permissive temperature continues to be more efficient than that by wild type. Since dif­ ferent adsorption rates of the phages may be ruled out by experiment, one is left with the possibility that wild type and mutant lysates produced under parallel conditions contain different fractions of transducing particles. Considering the protective function of the cap­ sule, the question remains to be answered how infec­ tion of cells which were not artificially decapsulated is possible. Experiments of W a r r e n and G r a y 11 indicate that streptococci which produce hyaluronic acid also elaborate hyaluronidase. Coexistence of the enzyme and its substrate leads to disappearance of hyaluronic acid on continued incubation. Since cells to be transduced came from the stationary phase of growth, they are likely to possess no pro-